Fast Five Quiz: HIV Recognition and Diagnosis

Michael Stuart Bronze, MD


February 16, 2023

Initial testing for HIV should be performed with an antigen-antibody immunoassay that detects HIV-1 antibodies, HIV-2 antibodies, and HIV-1 p24 antigen to test for established infection and acute HIV-1 infection. No further testing is required if initial immunoassay results are nonreactive.

Specimens that are reactive on the initial immunoassay and nonreactive or indeterminate on HIV-1/HIV-2 antibody differentiation should be investigated using nucleic acid testing. Specimens with a reactive immunoassay result should be tested with a supplemental antibody immunoassay that differentiates HIV-1 antibodies from HIV-2 antibodies.

Learn more on the workup of HIV.


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